Published 2012
| Version v1
Dataset
Open
UU 2803- A413T delta P1P2
Contributors
Researcher:
Description
E.coli mutant A413T (locked in the CheA off state) with P1P2 deletions in CheA
Technical info
Tilt Series Date: 2012-08-30
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSF tomo, IMOD, tomo3D
Collaborators and Roles: John S. Parkinson
Purification / Growth Conditions / Treatment: cells grown to log phase in TB medium, lysed with penicillin for 1 hour
Sample Preparation: plunge frozen in R2/2 Quantifoil grids in liquid ethane/propane
Attached Files
2803atlas10020.mrc, Tilt Series (Pixel Size 0.33 nm), 3.7 GB
2803atlas10020_full.rec, Reconstruction (Pixel Size 0.66 nm), 2.8 GB
Files
keyimg_aba2012-08-30-20.jpg
Additional details
Identifiers
- DOI
- 10.22002/md5rq-fdk68
- TILT_SERIES_ID
- aba2012-08-30-20
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-08-30
- Created
-
2012-08-31
- Updated
-
2012-11-19