hns_overexpression_PS152
Description
Technical info
Tilt Series Date: 2016-08-24
Data Taken By: Poorna Subramanian
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 170.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: grown for 27h from glycerol stock. At OD 0.51 induced with ~3mM IPTG and grown for 4h and froze as such, no spinning. OD was 0.52. Cell density and distribution looked good.
Attached Files
hnsM9_PS152_atlas10006.mrc, Tilt Series (Pixel Size 0.6 nm), 2.3 GB
hnsM9_PS152_atlas10006_full.rec, Reconstruction (Pixel Size 2.4 nm), 712.7 MB
psa2016-08-24-48_slicer17033.png, 359.0 kB
Files
keyimg_psa2016-08-24-48.jpg
Additional details
Identifiers
- DOI
- 10.22002/mg4vp-5hh26
- TILT_SERIES_ID
- psa2016-08-24-48
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-08-24
- Created
-
2016-08-25
- Updated
-
2016-09-01