Myxococcus xanthus MT299
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-08-19
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu Finder
Attached Files
20130819_MT299_cryoLM_10008.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20130819_MT299_cryoLM_10008_part131_28.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2013-08-19-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/mgcrn-fm545
- TILT_SERIES_ID
- ycw2013-08-19-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-08-19
- Created
-
2013-08-19
- Updated
-
2013-08-29