Caltech Tomography Archive
Published 2012 | Version v1
Dataset Open

MEFs (Rho-) Chan Lab

Woodward, Cora1
  • 1. ROR icon California Institute of Technology

Description

Mef cell line (Rho-) Chan Lab

Files

keyimg_cwl2012-05-29-26.jpg
Files (25.8 MB)
Name Size
keymov_cwl2012-05-29-26.mp4
md5:1143ac519d4973944127c827b143025b
24.6 MB Download
keyimg_cwl2012-05-29-26.jpg
md5:a9ef2dcd26e7dc6333eaa3fbdc95a253
1.2 MB Preview Download

Technical info

Tilt Series Date: 2012-05-29

Data Taken By: Cora Woodward

Species / Specimen: Murine embryonic fibroblast (MEFs)

Strain: Primary cells

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids

Sample Preparation: manual blotting

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-26/3dimage_69411/

12may29_C135_1_MEFnomitoDNA_AT3BT10003_full.rec, Reconstruction (Pixel Size 2.0 nm), 2.9 GB Download

12may29_C135_1_MEFnomitoDNA_AT3BT10003.mrc, Tilt Series (Pixel Size 0.5 nm), 677.3 MB Download

Additional details

Identifiers Funding Dates
Created:
May 24, 2023
Modified:
August 29, 2025