Image: Paracingulin controls the junctional accumulation of nonmuscle myosin-2 in endothelial cells in vivo

Description

Extended Data Figure Legend: A) Validation of Crispr/Cas9-mediated deletion of CGNL1 in two distinct clonal lines of bEnd.3 cells by genomic sequencing. The WT sequence is shown on top. B) Validation of KO by immunoblotting analysis of the expression of CGNL1, NM2A, NM2B and ZO-1 in WT and CGNL1-KO bEnd.3 cells. Beta-tubulin was used as a loading control. Numbers on the left refer to migration of molecular weight standards.

Files

Additional details