Published 2011 | Version v1
Dataset Open

pLE6

Creators

  • 1. ROR icon California Institute of Technology

Description

e. coli from Larry Rothfield containing plasmid carrying WT MreB.Induced with 1mM IPTG for 2 hours starting at OD600=0.2.

Technical info

Tilt Series Date: 2011-11-05

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: uploadraptor

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: LB 37 degrees

Attached Files

20111105_ple6_1mM_20004.mrc, Tilt Series (Pixel Size 0.95 nm), 1.1 GB

20111105_ple6_1mM_20004_part.rec, Reconstruction (Pixel Size 1.9 nm), 1.0 GB

Files

keyimg_mst2011-11-05-4.jpg

Files (2.1 GB)

Name Size
1.1 GB (external) Download
1.0 GB (external) Download
md5:e0ec0189c94852f94ca314faf4804948
1.2 MB Preview Download
md5:1ef887f7ac3f6216105dcc946d1c3338
29.8 MB Preview Download

Additional details

Identifiers

DOI
10.22002/mqa4f-pp102
TILT_SERIES_ID
mst2011-11-05-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-11-05
Created
2011-11-11
Updated
2011-11-11