Published 2018
| Version v1
Dataset
Open
MEF cells
Contributors
Researcher:
Description
Raw data files of MEF cells
Technical info
Tilt Series Date: 2018-11-06
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: MEF cells
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -3.0 μm, magnification: 33000x.
Microscope: Caltech Titan Krios
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: tomo3D
Collaborators and Roles: Wei Yang grew and froze cells
Purification / Growth Conditions / Treatment: Quantifoil grids (R 2/2 gold) coated with 0.1 mg/ml fibronectin
Sample Preparation: 3min 10uM FCCP, plunge frozen and FIB milled
Attached Files
TS5.mrc, Tilt Series (Pixel Size 0.432 nm), 1.8 GB
TS5_bin4_SIRT20_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 769.7 MB
Files
keyimg_smk2018-11-06-12.jpg
Additional details
Identifiers
- DOI
- 10.22002/mr336-a4255
- TILT_SERIES_ID
- smk2018-11-06-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-11-06
- Created
-
2019-04-01
- Updated
-
2019-04-02