Published 2015 | Version v1
Dataset Open

stable HAMP mutant

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

UU2612 with stable HAMP

Technical info

Tilt Series Date: 2015-03-12

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain: RP 437

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 8.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Sandy Parkinson University of Utah

Purification / Growth Conditions / Treatment: grown in TB plus gent, Amp, 50 µl 10mM IPTG overnight, penicillin lysis

Attached Files

stableHAMPgrid20004.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

stableHAMPgrid20004_part121_56.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

keyimg_aba2015-03-12-11.jpg

Files (4.2 GB)

Name Size
md5:0696fe4b10ff7e87417f32d7be9bae24
1.1 MB Preview Download
md5:1409d40c2655bcdac738ab451d262960
14.9 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/mtyxj-kyg83
TILT_SERIES_ID
aba2015-03-12-11

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-12
Created
2015-03-12
Updated
2015-09-21