Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

immunogoldMtrc_wt

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is wt made in perfusion chamber and fixed with 2.5% glutaraldehyde and immune-gold labeled for Mtr complex. The grid # is PS123-#3

Technical info

Tilt Series Date: 2015-12-29

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Batchruntomo

Collaborators and Roles: Sahand and Moh, USC

Sample Preparation: The sample was pulled out of Dh2O, was loaded on vitrobot with C side on left. Before blotting added 4ul of water. Blotting conditions: blot force -6, blot time -3.

Attached Files

atlas2_sq150018.rec, Reconstruction (Pixel Size 1.28 nm), 889.0 MB

atlas2_sq150018.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Files

keyimg_psa2015-12-29-18.jpg

Files (4.4 GB)

Name Size
3.4 GB (external) Download
889.0 MB (external) Download
md5:c60a932babdac30ac1496e72cac2fb76
1.0 MB Preview Download
md5:177af2e9973bd743ea035e5a5967225f
15.4 MB Preview Download

Additional details

Identifiers

DOI
10.22002/mv5wg-zmg21
TILT_SERIES_ID
psa2015-12-29-18

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-12-29
Created
2015-12-31
Updated
2016-01-04