Caltech Tomography Archive
Published 2020 | Version v1
Dataset Open

P. gingivalis wild type

Chreifi, Georges1
  • 1. ROR icon California Institute of Technology

Citation

Chreifi, G. (2020). P. gingivalis wild type [Data set]. CaltechDATA. https://doi.org/10.22002/my3h3-j7185

Description

Type IX secretion system, t9ss

Files

keyimg_gca2020-10-16-137.jpg
Files (8.7 MB)
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keymov_gca2020-10-16-137.mp4
md5:15bba58d8a4386747ee9c8a41daa9ca5
7.0 MB Download
keyimg_gca2020-10-16-137.jpg
md5:82b339204260a3e12c16a02837b867ee
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Technical info

Tilt Series Date: 2020-10-16

Data Taken By: Georges Chreifi

Species / Specimen: Porphyromonas gingivalis

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Eric Reynolds

Purification / Growth Conditions / Treatment: Cells grown anaerobically on a TSA+BHI plate at 37C for 3-4 days.

Sample Preparation: Harvested directly from plates, resuspended in Tris buffer, fiducials added, then plunge-frozen.. Vitrobot (MK iv) settings: 100% humidity, 3ul sample, Blot force 3, blot time 3.5 sec. Wait time 0 . sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Acquired using the FISE . method.

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2020-10-16-137

PginWT_8_135.mrc, Tilt Series (Pixel Size 0.333 nm), 1.8 GB Download

PginWT_8_135_part39_1.rec, Reconstruction (Pixel Size 1.332 nm), 884.7 MB Download

Additional details

Identifiers Funding Dates
Created:
June 16, 2023
Modified:
June 16, 2023