Published 2017 | Version v1
Dataset Open

E.coli minicell/Pul T2SS

Creators

  • 1. ROR icon California Institute of Technology

Description

grid box YWC252strain PAP5359 (minCDE knockout)Cells were grown on plate before freezing

Technical info

Tilt Series Date: 2017-05-15

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain provided by Ingrid Guilvout in Olivera Francetic lab

ycw2017-05-15-35_slicer18101.jpg: T2SS pseudopilus? ycw2017-05-15-35_slicer18102.jpg: T2SS pseudopilus?

Attached Files

20170515_YWC252b_10038.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

20170515_YWC252b_10038_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB

Files

keyimg_ycw2017-05-15-35.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:34f3eecf94bc66ff3f2ba99cce56d81b
1.0 MB Preview Download
md5:58c8838819c701a312c3c27b63e556b5
17.7 MB Preview Download
md5:a26c6d2c99227e68ad414b2d9c1d5e44
368.8 kB Preview Download
md5:efa38d556e30697b4039ba11feface1d
410.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/n20wk-bjx40
TILT_SERIES_ID
ycw2017-05-15-35

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-05-15
Created
2017-05-16
Updated
2017-05-30