Staphylococcus aureus_WT

Description

Technical info

Tilt Series Date: 2017-06-29

Data Taken By: Debnath Ghosal

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal.

Sample Preparation: Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot

Attached Files

Saureus_WT_06290003.mrc, Tilt Series (Pixel Size 0.39 nm), 1.9 GB

Saureus_WT_06290003_part35_6.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB