Saccharomyces cerevisiae - JAWY1

Description

Technical info

Tilt Series Date: 2016-11-30

Data Taken By: Shrawan kumar Mageswaran

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: SerialEM

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.

Purification / Growth Conditions / Treatment: Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-11-30-1to5,*-10,*-13to20 (TS1-TS14)- Heat shocked @38 deg C for 2min10min. sm2016-11-30-6to9,*-11to12 (TS15-21) - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.

Attached Files

TS3.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB

TS3_4bin_DB.rec, Reconstruction (Pixel Size 0.0 nm), 444.5 MB