HupAB knockout_M9_PS151

Description

Technical info

Tilt Series Date: 2016-08-24

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: grown for 31h from glycerol stock. Froze when OD was 0.41 as such, no spinning. Cell density and distribution looked good.

Attached Files

hupABM9_atlas10011.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

hupABM9_atlas10011_part121_4.rec, Reconstruction (Pixel Size 2.4 nm), 534.5 MB

psa2016-08-24-15_slicer17041.png, (diffuse blobs and pancake vesicles near the periplasm were seen in other strains of E.coli grown in minimal M9 media. I don't know how common this is in E.coli -andrew), 321.7 kB