Pombe Cryosections
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-06-03
Data Taken By: Matt Swulius
Species / Specimen: Schizosaccharomyces pombe
Strain: cdc25-22 rlc1-3GFP
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Manual/Raptor
Collaborators and Roles: Mithilesh Mishra, Mohan Balasubramanian
Purification / Growth Conditions / Treatment: Synchronized by 4 hr temp shift to 36 C then released to grow at 25 C. At 45 min I begin monitoring ring formation/constriction every 5-10 minutes until rings the majority of the rings were around mid-division.
Attached Files
060315_pombe_cryosection2_square5_tomo0001.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
060315_pombe_cryosection2_square5_tomo0001.rec, Reconstruction (Pixel Size 2.0 nm), 890.9 MB
Files
keyimg_mst2015-06-03-17.jpg
Additional details
Identifiers
- DOI
- 10.22002/n9n1b-nkh78
- TILT_SERIES_ID
- mst2015-06-03-17
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-06-03
- Created
-
2015-06-04
- Updated
-
2015-06-08