V.cholerae
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-01-06
Data Taken By: Poorna Subramanian
Species / Specimen: Vibrio cholerae
Strain: O1 biovar El Tor
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Yi-wei
Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.
psa2017-01-06-1_slicer17788.jpg: vesicles with legs sticking out psa2017-01-06-1_slicer17789.jpg: leaky DNA? psa2017-01-06-1_slicer17790.jpg: straight strands of DNA? psa2017-01-06-1_slicer17791.jpg: DNA? ladder structure? See a lot of these V. cholerae lysed cells
Attached Files
atlas30002_part121_60.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
atlas30002.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
Files
keyimg_psa2017-01-06-1.jpg
Files
(4.2 GB)
| Name | Size | |
|---|---|---|
| 3.4 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:b20fd860555a0d2b86f685cab8af3c7c
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1.1 MB | Preview Download |
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md5:66223459aa32410a79f1dac1493442c0
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15.6 MB | Preview Download |
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md5:4aae8241f4f425f88416a0b50da19a3a
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643.4 kB | Preview Download |
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md5:2ecc328800b4790394b3edf6708783ea
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635.3 kB | Preview Download |
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md5:2b56d2ce377c55b7f291f244349e37b2
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650.5 kB | Preview Download |
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md5:6c83bf8807e425bea91ff27647e4172b
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647.2 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/nat89-aba18
- TILT_SERIES_ID
- psa2017-01-06-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-01-06
- Created
-
2017-01-07
- Updated
-
2017-04-05