Xanthomonas vs E.coli (Br)

Description

Technical info

Tilt Series Date: 2017-03-08

Data Taken By: Debnath Ghosal

Species / Specimen: Xanthomonas citri

Strain: pv citri 306

Tilt Series Settings: Single Axis, tilt range: (-52.0°, 52.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Chuck Farah lab made the strains, data collected by Debnath Ghosal.

Purification / Growth Conditions / Treatment: 4 µL mixture of Xac and E.coli (Br) cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 15 min before freezing.

Sample Preparation: Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 6 sec

Attached Files

Xac_wt_20170308_V4_tgt00010003.mrc, Tilt Series (Pixel Size 0.39 nm), 2.0 GB

Xac_wt_20170308_V4_tgt00010003_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB