Published 2013 | Version v1
Dataset Open

HUVECs infx pNLGFP

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 150/A2. defocus: -6um. magnification: 19000.

Technical info

Tilt Series Date: 2013-07-03

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 19000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: pNLGFP obtained from Irvin Chen UCLA)

Sample Preparation: Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection

Attached Files

13jul03_HUVECspnl24hPI_montage_33x33_0041.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB

13jul03_HUVECspnl24hPI_montage_33x33_0041_full.rec, Reconstruction (Pixel Size 2.24 nm), 602.8 MB

Files

keyimg_cwl2013-07-03-40.jpg

Files (4.0 GB)

Name Size
3.3 GB (external) Download
602.8 MB (external) Download
md5:1037785435799da4ce7ae810f22affc9
967.5 kB Preview Download
md5:3e976ff3c1a62bfd8e2c2e098791dd6f
20.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/nmq9z-9hv06
TILT_SERIES_ID
cwl2013-07-03-40

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-07-03
Created
2013-07-05
Updated
2019-03-06