Published 2012
| Version v1
Dataset
Open
B. subtilis (2060)
Description
Cryosections of B. subtilis from Jeff Errington. Strain 2060 contains the c-myc tagged MreB under a xylose inducible promoter and is the original strain used in the 2001 paper.
Technical info
Tilt Series Date: 2012-08-27
Data Taken By: Matt Swulius
Species / Specimen: Bacillus subtilis
Strain:
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: autoPatch
Sample Preparation: cryosections of pelleted BS2060. Grown in LB with 0.25% xylose.
Attached Files
082712_BS2060d0037.mrc, Tilt Series (Pixel Size 0.493 nm), 3.7 GB
082712_BS2060d0037.rec, Reconstruction (Pixel Size 1.972 nm), 1.4 GB
Files
keyimg_mst2012-08-27-16.jpg
Additional details
Identifiers
- DOI
- 10.22002/nqx3f-p6s79
- TILT_SERIES_ID
- mst2012-08-27-16
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-08-27
- Created
-
2014-06-16
- Updated
-
2014-06-17