Published 2013 | Version v1
Dataset Open

HUVECs 24h PI pNLBglI

  • 1. ROR icon California Institute of Technology

Description

single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 150/A2. defocus: -6um. magnification: 6500

Technical info

Tilt Series Date: 2013-04-30

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 6500x.

Microscope: UCLA Titan

Acquisition Software: FEIbatchtomo

Upload Method: pipeline

Processing Software Used: FEIbatchtomo

Collaborators and Roles: Collected at UCLA with FEI Batchtomo

Sample Preparation: HUVECs imaged 24 hours after infection with pNLBglI. The goal is to image maturing HIV at the edge of infected cells.

Attached Files

13apr26_tomo_26.mrc, Tilt Series (Pixel Size 1.35 nm), 1.0 GB

13apr26_tomo_26_full.rec, Reconstruction (Pixel Size 2.7 nm), 629.1 MB

Files

keyimg_cwl2013-04-30-20.jpg

Files (1.7 GB)

Name Size
1.0 GB (external) Download
629.1 MB (external) Download
md5:e3d9c3d5b39314f1bfe9b93dab6bba61
1.2 MB Preview Download
md5:81c87883b96b23825ba8c57dcda68394
20.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/nsm4b-z2825
TILT_SERIES_ID
cwl2013-04-30-20

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-04-30
Created
2013-04-30
Updated
2019-03-06