Published 2015 | Version v1
Dataset Open

Mycobacterium marinum

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Grown on 2x LB to induce sporulation

Technical info

Tilt Series Date: 2015-10-12

Data Taken By: Elitza Tocheva

Species / Specimen: Mycobacterium marinum

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Will DePas from Dianne Newman's lab grew the cells

Sample Preparation: Cells were frozen with Vitrobot Mark 4. Grids were positively glow discharged.

Attached Files

Mmarinum_oct20150021_part121_5.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Mmarinum_oct20150021.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

Files

keyimg_etl2015-10-12-21.jpg

Files (4.0 GB)

Name Size
md5:eb596160d2e878ebe288ca01174c4264
986.7 kB Preview Download
md5:201428729656e1062b2e170f6c1f44e6
16.2 MB Preview Download
3.4 GB (external) Download
534.5 MB (external) Download

Additional details

Identifiers

DOI
10.22002/ntp71-21767
TILT_SERIES_ID
etl2015-10-12-21

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-10-12
Created
2015-10-13
Updated
2019-02-14