Published 2009 | Version v1
Dataset Open

Hyphomonas EB session21 34KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-07-14

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: raptor

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in PYE media, EB 0.02mg/ml overnight 4degree...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

jsa2009-07-14-102_slicer8944.jpg: Hyphomonas neptunium . DNA treated with EtBr?

Attached Files

09jul14a_b200c_103.mrc, Tilt Series (Pixel Size 0.632 nm), 511.7 MB

09jul14a_b200c_103_part.rec, Reconstruction (Pixel Size 1.264 nm), 367.0 MB

Files

jsa2009-07-14-102_slicer8944.jpg

Files (890.8 MB)

Name Size
511.7 MB (external) Download
367.0 MB (external) Download
md5:83380c5c24a407db4e227590f1b36c9e
323.2 kB Preview Download
md5:5bcd5ddcf865b7af53841580944d4e24
1.1 MB Preview Download
md5:1abae7ed0d9e6380cc95112e78ef903d
10.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/ntse2-gj887
TILT_SERIES_ID
jsa2009-07-14-102

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-07-14
Created
2009-10-20
Updated
2014-07-29