Myxococcus xanthus DK1622 wt
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-08-26
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200
ycw2013-08-26-75_slicer9104.jpg: T6SS, Myxococcus Xanthus ycw2013-08-26-75_slicer9105.jpg: pili ,Myxococcus Xanthus
Attached Files
20130826_DK1622_WT_30016.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20130826_DK1622_WT_30016_part131_4.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2013-08-26-75.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:b994ccf7a5ff7a1b7e8b3c427aac7e0b
|
1.1 MB | Preview Download |
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md5:c82fd6bd9a413ee9ccb002210711ef84
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26.6 MB | Preview Download |
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md5:d53fa72106d60f259569fa30aacf5488
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376.4 kB | Preview Download |
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md5:d954575ccc9356bd3d0096c945f0725c
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346.1 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/nv3vj-pkn58
- TILT_SERIES_ID
- ycw2013-08-26-75
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-08-26
- Created
-
2013-08-29
- Updated
-
2014-07-31