Published 2015 | Version v1
Dataset Open

HeLa cells

  • 1. ROR icon California Institute of Technology

Description

Synchronized HeLa cells, looking for midbodies

Technical info

Tilt Series Date: 2015-01-15

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: UCSF tomo

Sample Preparation: Synchronized HeLa cells with siRNA NUP 153 and Thymidine 2mM

Attached Files

helayfp60002.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

helayfp60002_full.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2015-01-15-42.jpg

Files (4.1 GB)

Name Size
3.4 GB (external) Download
623.6 MB (external) Download
md5:e2339a5f9258f6634201f2997cfc2127
1.1 MB Preview Download
md5:1f4000aafc4ac9a9552e7df23507ff1b
21.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/nv5b5-zez06
TILT_SERIES_ID
rra2015-01-15-42

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-01-15
Created
2015-01-20
Updated
2015-01-27