Thermococcus kodakarensis-cold

Description

Technical info

Tilt Series Date: 2017-01-13

Data Taken By: Debnath Ghosal

Species / Specimen: Thermococcus kodakarensis

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal

Purification / Growth Conditions / Treatment: Storing in a cold room for 2 months

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

dga2017-01-13-7_slicer17284.jpg: red: polyhedron . green: membrane connection between a mother cell and a membrane vesicle dga2017-01-13-7_slicer17285.jpg: polyhedron dga2017-01-13-7_slicer18129.jpg: Thermococcus kodakaraensis (cold-shock sample) . archaellum basal body (top view ?) . Ki Woo dga2017-01-13-7_slicer18130.jpg: Thermococcus kodakaraensis (cold-shock sample) . anchoring to the cone below the archaellum ? . . Ki Woo

Attached Files

Tkcoldroom-b0004_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

Tkcoldroom-b0004.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB