Published 2012 | Version v1
Dataset Open

NHP2L1 siRNA virus particles

  • 1. ROR icon California Institute of Technology

Description

HIV particles from producer cells treated with NHP2L1 siRNA

Technical info

Tilt Series Date: 2012-09-17

Data Taken By: Cora Woodward

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Gaelle Mercenne (Sundquist lab) generated virus. Viral particles were shipped to Caltech to be frozen and imaged by Cora Woodward

Purification / Growth Conditions / Treatment: Virus made in presence of NHP2L1 siRNA

Sample Preparation: manual blotting

Attached Files

12sep17_c141_2_AT1BT1_0002_BIN_full.rec, Reconstruction (Pixel Size 2.0 nm), 519.4 MB

12sep17_c141_2_AT1BT1_0002.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB

c141_2_0002_particle_tomo2_1_2.tiff, file associated to 3D Image #69445, 1.5 MB

Files

c141_2_0002_particle_tomo2_1_2.tiff

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Files (4.0 GB)

Name Size
3.4 GB (external) Download
519.4 MB (external) Download
1.5 MB (external) Download
md5:66d2baee834afc489c01daf7e48aa3d7
889.2 kB Preview Download
md5:3e2649830fb451cafffa9d74b0173ec5
8.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/p0cfm-41k32
TILT_SERIES_ID
cwl2012-09-17-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-09-17
Created
2015-09-16
Updated
2015-09-16