Bdellovibrio/Vibrio cholerae
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2013-05-01
Data Taken By: Yi-Wei Chang
Species / Specimen: Bdellovibrio bacteriovorus
Strain: HD100
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Acquisition Software: UCSF Tomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor
Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
Attached Files
grid3-tiltseries0022.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
grid3-tiltseries0022_part131_4.rec, Reconstruction (Pixel Size 1.68 nm), 516.7 MB
Files
keyimg_ycw2013-05-01-22.jpg
Additional details
Identifiers
- DOI
- 10.22002/p0tw5-tvw66
- TILT_SERIES_ID
- ycw2013-05-01-22
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-05-01
- Created
-
2013-05-03
- Updated
-
2013-05-24