Published 2014 | Version v1
Dataset Open

Vibrio deltaCheA2

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

V. cholerae strain deleted for CheA2

Technical info

Tilt Series Date: 2014-04-10

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

aba2014-04-10-4_slicer3816.jpg: Delta CheA2 short membranebound array

Attached Files

VibrioDeltaA20002.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioDeltaA20002_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

aba2014-04-10-4_slicer3816.jpg

Files (4.2 GB)

Name Size
md5:055e52bcb3f2563ec98797e312d30124
274.0 kB Preview Download
md5:8e0b619aab0092dc437819fdb7778302
1.1 MB Preview Download
md5:aa12f929e1878e4f0baee50ff17ca478
26.1 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/p3r6y-6yz81
TILT_SERIES_ID
aba2014-04-10-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-10
Created
2014-04-10
Updated
2014-09-24