Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

Mega gas vesicles

Creators

  • 1. ROR icon California Institute of Technology

Description

Mega gas vesicles, purified from E. coli, -1 to -5 micron defocus. Reconstruct with included scripts for ctf-corrected bin1 tomogram.

Technical info

<strong>Tilt Series Date:</strong> 2019-09-25</p>

<strong>Data Taken By:</strong> LaurenAnn Metskas</p>

<strong>Species / Specimen:</strong> Bacillus megaterium</p>

<strong>Strain:</strong> </p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-42.0°, 45.0°), step: 3°, constant angular increment, dosage: 70.0 eV/Ų, defocus: 0.0 μm, magnification: 81000x. </p>

<strong>Acquisition Software:</strong> Serial EM</p>

<strong>Upload Method:</strong> rundir</p>

<strong>Processing Software Used:</strong> IMOD 4.9.8, ctffind4, custom scripts</p>

<strong>Collaborators and Roles:</strong> purified by Rob Hurt and George Lu from the Shapiro lab at Caltech; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas with assistance from Przemek Dutka</p>

<strong>Purification / Growth Conditions / Treatment:</strong> purified Mega gas vesicles in 10 mM HEPES</p>

<strong>Sample Preparation:</strong> protochips C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity</p>

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/rawdata/</p>

21_dose-filt.st, Tilt Series (Pixel Size 0.1104 nm), 2.8 GB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/rawdata/21_dose-filt.st" > <i class="download icon"></i> Download </a>

</p>

21_dose-filt.rec, Reconstruction (Pixel Size 0.8832 nm), 331.8 MB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/3dimage_121845/21_dose-filt.rec" > <i class="download icon"></i> Download </a>

</p>

TS_21.mrc.mdoc, 28.1 kB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/rawdata/TS_21.mrc.mdoc" > <i class="download icon"></i> Download </a>

</p>

frames.tar.bz2, zipped raw frames, 1.6 GB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/file_27744/frames.tar.bz2" > <i class="download icon"></i> Download </a>

</p>

scripts.tar.bz2, zipped processing scripts, 18.6 kB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-09-25-22/file_27745/scripts.tar.bz2" > <i class="download icon"></i> Download </a>

</p>

Files

keyimg_lma2019-09-25-22.jpg

Files (6.2 MB)

Name Size
md5:0e32501d0201678ed9af0a1f6e962781
424.1 kB Preview Download
md5:c538f4d3a021717693627729ca7a9516
5.8 MB Preview Download

Additional details

Identifiers

DOI
10.22002/p9eqz-fhz32
TILT_SERIES_ID
lma2019-09-25-22

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2019-09-25
Created
2021-06-29
Updated
2021-06-29