JV9082_K3_janelia

Description

Technical info

<strong>Tilt Series Date:</strong> 2019-12-04</p>

<strong>Data Taken By:</strong> Debnath Ghosal</p>

<strong>Species / Specimen:</strong> Legionella pneumophila</p>

<strong>Strain:</strong> Philadelphia-1_Lp02</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Ų, defocus: -3.0 μm, magnification: 26000x. </p>

<strong>Microscope:</strong> Janelia Titan</p>

<strong>Acquisition Software:</strong> SerialEM</p>

<strong>Upload Method:</strong> pipeline</p>

<strong>Processing Software Used:</strong> Raptor</p>

<strong>Collaborators and Roles:</strong> Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.</p>

<strong>Purification / Growth Conditions / Treatment:</strong> Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.</p>

<strong>Sample Preparation:</strong> Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.</p>

External Files

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