HeLa cells expressing TRIM5α-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2016-05-05
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Tom Hope
Purification / Growth Conditions / Treatment: Grown on R2/2 London finder grids coated with fibronectin
Sample Preparation: Manual Blotting
Attached Files
tomography5.mrc, Tilt Series (Pixel Size 6.167 nm), 3.0 GB
tomography5.rec, Reconstruction (Pixel Size 24.668 nm), 1.8 GB
tomographynewstack_full.rec, Reconstruction (Pixel Size 0.0 nm), 668.2 MB
Files
keyimg_scd2016-05-05-5.jpg
Additional details
Identifiers
- DOI
- 10.22002/pewbp-z1619
- TILT_SERIES_ID
- scd2016-05-05-5
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-05-05
- Created
-
2021-03-12
- Updated
-
2021-03-30