Caltech Tomography Archive
Published 2016 | Version v1
Dataset Open

Myxococcus xanthus SA6888

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

Strain SA6888 (minor pilin cluster 1+2 deletion, Δ mxan1021-1017; Δmxan0364-0359).Grid box YWC224

Technical info

Tilt Series Date: 2016-09-15

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3.0°, constant angular increment, dosage: 80.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics.

Sample Preparation: Vitrobot (MK vi) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu Finder.

Attached Files

20160915_YWC224_10011.mrc, Tilt Series (Pixel Size 0.39 nm), 1.2 GB

20160915_YWC224_10011_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB

Files

keyimg_ycw2016-09-15-11.jpg

Files (1.7 GB)

Name Size
1.2 GB (external) Download
534.5 MB (external) Download
md5:0be2a9d103b5b5c35f310a4271957549
1.1 MB Preview Download
md5:7a53283dd5d45c1c79438a87158d38d3
18.1 MB Preview Download

Additional details

Identifiers

DOI
10.22002/pm0pq-gsw46
TILT_SERIES_ID
ycw2016-09-15-11

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-09-15
Created
2016-09-15
Updated
2016-09-16