The compressed .tar.gz file contains all raw images used for Figures 3 and 5 of the baseMEMOIR paper. It consists of a folder "Images", which has subfolders for each hybcycle (note that these are 0 indexed; for example, hyb cycle 50 corresponds to the membrane staining round indicated in "Imaging_Hybridization_Rounds.xlsx", which can be found in the main supplementary data folder). Each of these folders contains raw z-stack images for each position ("MMStack_Pos0.ome.tif") and a subfolder containing max projections across the z-stacks for each position ("HybCycle_XX_Max").


There are also additional folders: 
- "PoorFocus", containing the imaging results from round 0, which were not good quality and were not used for subsequent analysis
- "HybCycle_reimage_6", which contains repeated images for HybCycle 6. During imaging, the microscope encountered an error during the original HybCycle 6. I restarted the microscope, repeating the entire round. For analysis, I used all original HybCycle 6 images except for positions 9 and 10, for which I used the new images (see also the note in the HybCycle_6 folder)
- "Pre_HybCycle_XX" folders contain images collected after washing away secondary readout probes for some rounds to verify that signal was not retained over the course of the experiment