S. cerevisiae - 6210 + pAM21
Description
Technical info
Tilt Series Date: 2016-10-07
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Babst Lab (Markus Babst and Akshay Moharir) - Provided strain with the plasmid transformed.
Purification / Growth Conditions / Treatment: 6210 + pAM21(Vps36-V5-Strep in Ura plasmid). Grown to saturation overnight and cut back to 0.2 OD and grown until 0.6 OD. Media -> -URA YNB
Sample Preparation: Pull down of P13 fraction using Dynabeads® M-280 Streptavidin from 5801D trial kit. IN fraction (Input fraction for Dynabeads - see Jensen Lab Protocols page 14).
Attached Files
IN_TS23.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB
IN_TS23_4bin_DB.rec, Reconstruction (Pixel Size 0.0 nm), 199.1 MB
Files
IN_TS23_SS_DB.jpg
Additional details
Identifiers
- DOI
- 10.22002/png8x-t7j78
- TILT_SERIES_ID
- smk2016-10-07-25
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-10-07
- Created
-
2017-01-17
- Updated
-
2017-01-25