Published 2011
| Version v1
Dataset
Open
HUVECs 24h Infx pNLgfp
Description
HUVECs infected by spinoculation with VSVG-pNL4GFP, frozen 24 h after synchronized entry
Technical info
Tilt Series Date: 2011-03-31
Data Taken By: Cora Woodward
Species / Specimen: HUVECs
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: pNL4GFP provirus construct from Irvin Chen (UCLA). Cells infected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected
Sample Preparation: manual blotting
Attached Files
11mar31a__047.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB
11mar31a__047_full.rec, Reconstruction (Pixel Size 2.0 nm), 5.1 GB
Files
keyimg_cwl2011-03-31-36.jpg
Additional details
Identifiers
- DOI
- 10.22002/ppesf-yfw78
- TILT_SERIES_ID
- cwl2011-03-31-36
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-03-31
- Created
-
2015-09-16
- Updated
-
2015-09-16