V. cholerae RT4236 (refreshed)

Description

Technical info

Tilt Series Date: 2015-07-13

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: O395-N1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain from Ronald K. Taylor

Purification / Growth Conditions / Treatment: Overnight cultures of Vibrio cholerae O395 N1 pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin. Refreshed in fresh media right before freezing.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: C-flat Cu 2/2.

Attached Files

20150713_AK15_10024.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

20150713_AK15_10024_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB