Published 2014 | Version v1
Dataset Open

HeLa cells

  • 1. ROR icon California Institute of Technology

Description

Looking for ESCRT near midbodies

Technical info

Tilt Series Date: 2014-12-08

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: with Catherine Ullman and Doug mckay

Sample Preparation: siRNA NUP 153+Thymidine for 14h,

Attached Files

midbody20001.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

midbody20001_full.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2014-12-08-3.jpg

Files (4.1 GB)

Name Size
md5:cdbd8ead8996fc7b37f9035d8c24fa74
1.1 MB Preview Download
md5:e7b1f900ea6f68a40d214db5ac6c2da8
21.6 MB Preview Download
3.4 GB (external) Download
623.6 MB (external) Download

Additional details

Identifiers

DOI
10.22002/pv3sb-hz731
TILT_SERIES_ID
rra2014-12-08-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-12-08
Created
2014-12-09
Updated
2014-12-10