Published 2014 | Version v1
Dataset Open

HeLa cells

  • 1. ROR icon California Institute of Technology

Contributors

Description

Raw data files of HeLa cells

Technical info

Tilt Series Date: 2014-11-13

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: cells and siRNA obtained from Doug Mackay/ Cathrine Ullman

Sample Preparation: HeLa cells+ siRNA NUP153+ Thymidine 2mM, 14 h post thymidine release

rra2014-11-13-55_slicer10583.jpg: actin bundle rra2014-11-13-55_slicer10585.jpg: tube inside a tube rra2014-11-13-55_slicer10586.jpg: tube filled with actin?

Attached Files

midbody70008.mrc, Tilt Series (Pixel Size 0.6 nm), 3.2 GB

midbody70008_full.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2014-11-13-55.jpg

Files (3.8 GB)

Name Size
md5:bc74289088754bdf4c4c42e30a6d1f60
1.1 MB Preview Download
md5:516a39d7b343728edc8762908b02c6fc
21.0 MB Preview Download
3.2 GB (external) Download
623.6 MB (external) Download
md5:3dfca3c723b0cf8aae29bc579f5dc172
258.8 kB Preview Download
md5:0bbf615c58a208edfd998b615dfa060f
327.0 kB Preview Download
md5:0bfbeaf5535aa3e5fddd85513d37e6eb
234.1 kB Preview Download
md5:04a8d70b0c781b58e1c2aa43a3d7663d
397.1 kB Preview Download

Additional details

Identifiers

DOI
10.22002/pxz7c-fd221
TILT_SERIES_ID
rra2014-11-13-55

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-11-13
Created
2014-11-14
Updated
2014-11-17