Caulobacter crescentus

Description

Technical info

Tilt Series Date: 2006-09-20

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias

Purification / Growth Conditions / Treatment: ddi-water ATCC (PYE) media; cultured in 50ml flask; 7 days growth; see DPD notebook Sep 13-18, 2006

Sample Preparation: box 697, grid 3; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Sep 18, 2006

Attached Files

CC06Sep20t02.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB

CC06Sep20t02_18kx.mrc, Tilt Series (Pixel Size 1.2 nm), 8.4 MB