Xac WT pBRaφ pBRR MCS5 GFP
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2017-12-15
Data Taken By: Debnath Ghosal
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Chuck Farah lab made the strain, data collected by Debnath Ghosal.
Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 6 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.
Attached Files
XanGFP15122017b0026.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB
XanGFP15122017b0026.rec, Reconstruction (Pixel Size 1.56 nm), 889.0 MB
Files
keyimg_dga2017-12-15-98.jpg
Additional details
Identifiers
- DOI
- 10.22002/q4r40-nm352
- TILT_SERIES_ID
- dga2017-12-15-98
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-12-15
- Created
-
2017-12-18
- Updated
-
2018-08-29