Hyphomonas "AR37"
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-03-03
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees. Expression of Venus labeled HNK0708 was induced by adding final 300 uM CuCl2 and incubated at 30 degrees for 24 hr.
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
ycw2012-03-03-17_slicer8687.jpg: REZ/Nucleoid . Ribsome exclusion zone . Hyphomonas neptunium
Attached Files
2012Mar03_AR37_24hr_10014.mrc, Tilt Series (Pixel Size 0.78 nm), 1.0 GB
2012Mar03_AR37_24hr_10014_part122_58.rec, Reconstruction (Pixel Size 1.56 nm), 516.1 MB
Files
keyimg_ycw2012-03-03-17.jpg
Additional details
Identifiers
- DOI
- 10.22002/qbne7-z1g92
- TILT_SERIES_ID
- ycw2012-03-03-17
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-03
- Created
-
2012-03-04
- Updated
-
2014-07-28