Caltech Tomography Archive
Published 2006 | Version v1
Dataset Open

Caulobacter crescentus

Dias, Prabha1
  • 1. ROR icon California Institute of Technology
Researcher:
Prabha Dias, D.

Description

wild type CB15N. 7 of 7, some warping in lower-right quadrant, good contrast. skinny straight swarmer? cell; no obvious S-layer; no obvious filaments; amorphous medium-dense core without ribosomes, ~1/3 cell length; >5 very small storage granules

Files

pdd2006-09-20-7_slicer10065.jpg
Files (10.1 MB)
Name Size
pdd2006-09-20-7_slicer10065.jpg
md5:454fb8805d2ac5865a8f52b9e8b2c20c
262.0 kB Preview Download
keymov_pdd2006-09-20-7.mp4
md5:eff9a7b007dc3e582d26128a0cfcca67
8.7 MB Download
keyimg_pdd2006-09-20-7.jpg
md5:b6fa9ed0bef7aa4942610192e1cae013
1.0 MB Preview Download
SAVE_06Sep20t07_xy.jpg
md5:385fdb16b27fcd7370dbdc597d76f489
187.9 kB Preview Download

Technical info

Tilt Series Date: 2006-09-20

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.6°, 60.6°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Microscope: 0

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias

Purification / Growth Conditions / Treatment: ddi-water ATCC (PYE) media; cultured in 50ml flask; 7 days growth; see DPD notebook Sep 13-18, 2006

Sample Preparation: box 697, grid 3; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Sep 18, 2006

SAVE_06Sep20t07_xy.jpg: file associated to 3D Image #341 pdd2006-09-20-7_slicer10065.jpg: Caulobacter crescentus . REZ ribosome exclusion zone

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-09-20-7/rawdata/

CC06Sep20t07.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB Download

CC06Sep20t07_18kx.mrc, Tilt Series (Pixel Size 1.2 nm), 8.4 MB Download

06Sep20t07bn_full.rec, Reconstruction (Pixel Size 2.4 nm), 734.0 MB Download

SAVE_06Sep20t07_xy.tif, file associated to 3D Image #341, 2.9 MB Download

06Sep20t07.mov, file associated to 3D Image #341, 373.4 MB Download

Additional details

Identifiers Funding Dates
Created:
May 16, 2023
Modified:
August 22, 2025