Published 2011 | Version v1
Dataset Open

HUVECs 24h Infx pNLgfp

  • 1. ROR icon California Institute of Technology

Description

HUVECs infected by spinoculation with VSVG-pNL4GFP, frozen 24 h after synchronized entry

Technical info

Tilt Series Date: 2011-03-31

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: pNL4GFP provirus construct from Irvin Chen (UCLA). Cells infected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected

Sample Preparation: manual blotting

Attached Files

11mar31a__050_full.rec, Reconstruction (Pixel Size 2.0 nm), 4.8 GB

11mar31a__050.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

11mar31a__050_medflt.tiff, file associated to 3D Image #68473, 947.0 kB

Files

11mar31a__050_medflt.tiff

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Files (5.8 GB)

Name Size
1.0 GB (external) Download
4.8 GB (external) Download
947.0 kB (external) Download
md5:276e245cb47af25ffd8f1ddec2a2a9bb
4.7 MB Preview Download
md5:9afeb8947977192df0a07bf99c60b337
13.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/qmf64-kr580
TILT_SERIES_ID
cwl2011-03-31-37

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-03-31
Created
2015-09-16
Updated
2015-09-16