Cryo-sectioned Jurkat VSVG-pNL4GFP

Description

Technical info

<strong>Tilt Series Date:</strong> 2011-12-12</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> Jurkat Human T-cell</p>

<strong>Strain:</strong> Clone E6-1</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>

<strong>Acquisition Software:</strong> UCSF tomo</p>

<strong>Upload Method:</strong> rundir</p>

<strong>Processing Software Used:</strong> imod</p>

<strong>Collaborators and Roles:</strong> pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward</p>

<strong>Purification / Growth Conditions / Treatment:</strong> infected VSVG-pNL4GFP</p>

<strong>Sample Preparation:</strong> cryo-sectioned by Mark Ladinsky</p>

11dec12_cryosec_pnlegfp_grid3_Batch5_0043_full.jpg: file associated to 3D Image #54399

External Files

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