Rhesus Macaque Fibroblast
Description
Technical info
Tilt Series Date: 2015-04-20
Data Taken By: Stephen Carter
Species / Specimen: Rhesus Macaque Fibroblasts infected with Vpr-Ruby HIV-1
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Acquisition Software: UCSFTomo
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Fibroblasts VSV-G pseudotyped virus Vpr-Ruby provided by Tom Hope
Purification / Growth Conditions / Treatment: Cells infected with ZLK 106-06-06-14 HIV-1 GFP Vpr Ruby. 500 µl of media and 500 µl of virus. Virus spinoculated at 16°C at 18 g for 1 hr. Cells were washed with warm media and incubated at 37°C. Cells frozen at early time points approximately 20 mins after temperature shift.
Attached Files
tomography2.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
tomography2_full.rec, Reconstruction (Pixel Size 0.0 nm), 666.7 MB
Files
keyimg_scd2015-04-20-2.jpg
Additional details
Identifiers
- DOI
- 10.22002/qr58h-ge781
- TILT_SERIES_ID
- scd2015-04-20-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-04-20
- Created
-
2015-04-20
- Updated
-
2016-07-13