Caltech Tomography Archive
Published 2010 | Version v1
Dataset Open

Skinny E. coli with GFP-MreB

Li, Zhuo1
  • 1. ROR icon California Institute of Technology

Description

Raw data files of Skinny E. coli with GFP-MreB

Files

zla2010-03-05-12_slicer9502.jpg
Files (18.8 MB)
Name Size
zla2010-03-05-12_slicer9502.jpg
md5:0c90b2d3c35394efadcf23043c1f4714
380.7 kB Preview Download
keyimg_zla2010-03-05-12.jpg
md5:f1f8aa30ae235d66c9a73a06beceebb8
1.3 MB Preview Download
keymov_zla2010-03-05-12.mp4
md5:0bf4acf41359f345aeb72cca449deb0d
16.8 MB Download
10mar05a_ZL000_012low.jpg
md5:134e25b7086eb39f4ba5d53d2d43adba
314.1 kB Preview Download
10mar05a_ZL000_012ov.jpg
md5:ea703e5ddb41e6ac1d7777e37cfa5f2a
79.6 kB Preview Download

Technical info

Tilt Series Date: 2010-03-05

Data Taken By: Zhuo Li

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.

Purification / Growth Conditions / Treatment: L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)

Sample Preparation: R2/2 CF or Quantifoil, 10 nm gold, 100% humidity

zla2010-03-05-12_slicer9502.jpg: E. coli . Vesicle

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-12/rawdata/

10mar05a_ZL000_012.mrc, Tilt Series (Pixel Size 1.192 nm), 721.4 MB Download

10mar05a_ZL000_012_part.rec, Reconstruction (Pixel Size 2.384 nm), 524.3 MB Download

10feb18a_dJnofe2_012.mrc, Tilt Series (Pixel Size 1.192 nm), 1.1 GB Download

10feb18a_dJnofe2_012_full.rec, Reconstruction (Pixel Size 2.384 nm), 524.3 MB Download

Additional details

Identifiers Funding Dates
Created:
May 16, 2023
Modified:
August 25, 2025