Published 2012 | Version v1
Dataset Open

COS7 pNLGFP INF-alpha

  • 1. ROR icon California Institute of Technology

Contributors

Description

Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C110

Technical info

Tilt Series Date: 2012-01-20

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine

Sample Preparation: manual blotting

Attached Files

12jan20_C110_3_pNL_INFalpha_AT1BT10006_full.rec, Reconstruction (Pixel Size 2.0 nm), 2.9 GB

12jan20_C110_3_pNL_INFalpha_AT1BT10006.mrc, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

12jan20_C110_3_pNL_INFalpha_AT1BT10006_full.tiff, file associated to 3D Image #69062, 1.1 MB

Files

12jan20_C110_3_pNL_INFalpha_AT1BT10006_full.tiff

This file cannot be previewed
  • This file is an external reference and not stored directly in this repository. To access its content, please download it and open it locally.

Additional details

Identifiers

DOI
10.22002/qrqbh-9dp59
TILT_SERIES_ID
cwl2012-01-20-32

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-01-20
Created
2015-09-16
Updated
2015-09-16