V.cholerae
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-01-06
Data Taken By: Poorna Subramanian
Species / Specimen: Vibrio cholerae
Strain: O1 biovar El Tor
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Yi-wei
Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.
Attached Files
sq50005.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
sq50005_part121_47.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_psa2017-01-06-123.jpg
Additional details
Identifiers
- DOI
- 10.22002/qw8n7-bng10
- TILT_SERIES_ID
- psa2017-01-06-123
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-01-06
- Created
-
2017-01-10
- Updated
-
2017-04-05