Published 2019
| Version v1
Dataset
Open
Conjugation_Ecoli
Contributors
Researcher:
Description
These are conjugation in Ecoli samples. The cells are the ANB35 cells (donor) and ANB24 . cells (recipient), More information on these strains can be found in the paper (Direct . visualization of horizontal gene transfer). These are tomograms from (square 3) in Grid 1 . from box 4 (See William's notes for more information). There are cryoLM data for this grid . obtained during the Linkam stage demo.
Technical info
Tilt Series Date: 2019-06-23
Data Taken By: Mohammed Kaplan
Species / Specimen: Escherichia coli
Strain: ANB35, Hfr3000 mrfp1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: None
Collaborators and Roles: The samples were sent by Ariel B Lindner (who is the second author on the above-mentioned . paper) in Paris.
Attached Files
Square3Montagev2.mrc, Tilt Series (Pixel Size 0.449 nm), 437.1 MB
Square3Montagev2.mrc.mdoc, 25.9 kB
Files
keyimg_mka2019-06-23-30.jpg
Additional details
Identifiers
- DOI
- 10.22002/qwnvh-14522
- TILT_SERIES_ID
- mka2019-06-23-30
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-06-23
- Created
-
2019-06-25
- Updated
-
2022-10-15