E. coli (pLE7, 20 uM IPTG)
Description
Technical info
Tilt Series Date: 2011-06-02
Data Taken By: Matt Swulius
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFTomo
Collaborators and Roles: Gift from Larry Rothfield
Purification / Growth Conditions / Treatment: Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.
Sample Preparation: Vitrobot plunge freezing
mst2011-06-02-6_slicer9674.jpg: Escherichia coli . vesicle mst2011-06-02-6_slicer9676.jpg: Escherichia coli . filament bundle . contains MreB mst2011-06-02-6_slicer9678.jpg: Escherichia coli . vesicle
Attached Files
20110602_PLE7_11.mrc, Tilt Series (Pixel Size 0.95 nm), 1.0 GB
20110602_PLE7_11_full.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB
Files
keyimg_mst2011-06-02-6.jpg
Files
(1.9 GB)
| Name | Size | |
|---|---|---|
| 1.0 GB | (external) Download | |
| 825.8 MB | (external) Download | |
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md5:1caf65488614c6e6b94efe4b056bced9
|
1.2 MB | Preview Download |
|
md5:7f44867aefce35db4f64acd94cc8a6c9
|
25.7 MB | Preview Download |
|
md5:09874caea034b869e2ab43a349dae6d0
|
432.2 kB | Preview Download |
|
md5:e135c60f581c6629d7105503c81848dc
|
433.3 kB | Preview Download |
|
md5:6f02500210648fe28855013c87bfa6ac
|
430.9 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/qxag8-rx345
- TILT_SERIES_ID
- mst2011-06-02-6
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-06-02
- Created
-
2012-07-03
- Updated
-
2014-08-07